As a Research Assistant 2, this individual is responsible for contributing to ongoing research projects in the Center for Embryonic Cell and Gene Therapy (CECGT, http://www.ohsu.edu/xd/research/centers-institutes/embryonic-cell-gene-therapy-center/ ). A successful RA2 will perform a variety of duties, which require flexibility, judgment, and unique experience in the field of cell replacement therapy. This research center reports to the Chief Research Officer and is a critical scientific center at OHSU; thus, all employees are expected to communicate with high-level internal and external personnel and must operate with great accuracy and efficiency.
Research responsibilities include providing support to ongoing molecular biology and sequence derivation studies related to genetic, epigenetic and transcriptomic analyses of DNA (human, primate, mouse), gametes, embryos and pluripotent stem cells; participating in embryo and stem cell culture, mouse models including in vivo and in vitro experiments, cellular and molecular assays; and participating in the on-call lab schedule rotation (intermittent evening, weekend, and holiday coverage). The RA2 assists with purchasing of equipment and supplies and communicating with scientific and support staff. This position also contributes to written reports, abstracts, project proposals and grants, and presentations.
Seeking an RA2 whom will perform a variety of duties, which require flexibility, judgment, and unique experience in the field of cell replacement therapy. The Center for Embryonic Cell and Gene Therapy under the direction of Dr. Shoukhrat Mitalipov reports to the Chief Research Officer and is a critical scientific center at OHSU. Research responsibilities include: molecular biology and sequence derivation studies related to genetic, epigenetic and transcriptomic analyses of DNA (human, primate, mouse), gametes, embryos and pluripotent stem cells; participating in embryo and stem cell culture, mouse models including in vivo and in vitro experiments; and cellular and molecular assays. Please see our website for more information about our team! http://www.ohsu.edu/xd/research/centers-institutes/embryonic-cell-gene-therapy-center/ )
1. Research Molecular Biology and Sequence Derivation Perform various molecular techniques such as DNA/RNA/library preparation, PCR, ARMS-qPCR, PyroSequencing, Sanger sequencing, Next- Generation Sequencing (MiSeq, AmpliSeq, others), Immunocytochemistry, Karyotyping, and other assays as deemed necessary Gel preparation, loading, and imaging Assist with analysis of sequence information and data submissions to open source databases such as the NCBI as relevant Maintain/update software, computers, equipment, and materials associated with library preparation for NGS sequencing and analysis Maintain and coordinate with project leaders detailed databases of sequence data analyzed and performed in all aspects of research in the Center, including; human, rodent, non-human primate samples
2. Research Cell Culture and Animal Studies Assist in maintaining and derivation of fibroblast cultures Assist in maintaining ESC and iPS cell cultures, including media preparation Assist in animal-related research procedures such as mice tissue collection, derivation of mouse embryonic fibroblast cells Assist in mouse colony maintenance and weekend colony maintenance as needed, including animal injections
3. Research General Laboratory Perform general lab upkeep and record keeping as necessary Provide support to stocking laboratory and cell culture rooms Provide support in laboratory and cell culture room cleaning schedule, autoclaving, and decontamination
4. Research Data Collection/Data Analysis/Record Keeping Collect scientific data pertinent to the research projects, keep records of information obtained during research (which may include database searches) Maintain integrity of CECGT databases
5. Report Preparation and Literature Research Prepare reports containing analytical, descriptive, and evaluative content that will be submitted for publication or for contribution to grant applications, progress reports, presentations at national and international meetings, etc. Conduct research of literature and database analysis
6. Other Projects in conjunction with the mission of the Center for Embryonic Cell and Gene Therapy and/or OHSUs Research & Innovation Office
Bachelors Degree with major courses in field of research,ORAssociates Degree and 3 years of relevant work experience.
Knowledge of mitochondrial genetics and stem cell biology
Knowledge of NGS sequencing analysis and library preparation
Previous work with scientists in an academic environment
Appreciating and cultivating a diverse study population
Self-starter with excellent eye to detail and organization skills
Independent motivator, strong internal drive to succeed
Exhibits integrity and openness to learning
Masters Degree in relevant field
3 years of relevant research experience, with demonstrated research or clinical experience in molecular biology, cell biology and genetics with laboratory experience with sequencing, cell culture and NGS library preparation
Working conditions: Monday through Friday, commonly 8:00a 4:30p or 7:00a 3:30p position with weekend and holiday call coverage. May be required to work evenings or weekends to cover laboratory cell and embryo culture duties and laboratory animal duties. Willing and able to telework and/or work in person as directed by managing staff. Appointee will be in contact with human, mouse, and primate tissues and fluids and will be responsible for fluid and tissue collection as related to study protocol. Training will be provided for proper handling, processes, and procedures.
Physical demands & equipment usage: Ability to stand and be ambulatory for more than 6 hours. Able to lift 25 pounds. This position requires significant use of a pipette, repetitive hand motions, working at a computer; ability to perform key office functions including answering the phone, responding to e-mail, composing documents and responding to phone and e-mail inquiries. Ability to physically perform tasks involving handling, moving, or restraint of animals, collection of samples, administration of treatments, and other procedures (this may involve moving on wet and/or slick surfaces, through narrow corridors, between rows of cages). Position also requires walking to various schools/departments and possible delivery of materials at OHSU buildings off the main campus.
Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or email@example.com.
As the state's only health and research university, OHSU brings together patient care, research, education of the next generation of health care providers and scientists and community service to improve the health and well-being of all Oregonians.The university is a conduit for learning for more than 2,800 students, providing training to interns, residents and fellows in postgraduate medical and research. It is the only place in Oregon that grants doctoral degrees in medicine, dentistry and nursing. OHSU cares for more than 251,000 patients each year from around the region and takes a leadership role in contributing to solutions involving the health of the state and the nation, such as health care access and transparency, ethics and workforce shortage. Beyond providing critical health care services, quality education and cutting-edge research, OHSU also is a key economic and social force in the Northwest. With an annual operating budget of $2.06 billion and more than 14,000 employees, OHSU is Portland?s largest employer. Its size contributes to its ability to provide... many services and community support activities not found anywhere else in Oregon. For more information visit http://www.ohsu.edu/xd/about/index.cfm